JOIN US FOR HOMEWOOD FLOSSMOOR SCIENCE PUB!
Doors open 6pm
March 28th, 2017
Metagenomic Characterization of Bacterial Diversity and Succession in The Shedd Aquarium’s Wile Reef Shark Exhibit
Presented by: Tim Gsell, Professor of Chemistry, Governors State University
Dr. Gsell will present on the metagenomic characterization of bacterial diversity and succession from a series of samples taken during the set-up of the Wild Reef Shark Exhibit at the Shedd Aquarium. This study takes advantage of the use of previously collected samples from the quarantine tanks for the sharks, the filter systems used to continuously remove toxic nitrogenous waste from the water, and sand filters used for each tank system. The previous work focused on only the ammonia oxidizer bacterial populations. That work showed distinct patterns of Nitrosomonas and Nitrosopira genus types succession Shifts from Nitrosospira-like to Nitrosomonas-like sequences in one enrichment tank, and succession to predominantly Nitrosospira-like species after the support media was transferred to the aquarium’s life support system’s filters. The Nitrosospira-like sequences persisted as the dominant phylotype through the end of the study. The metagenomics work has confirmed the patterns shown earlier, but also focuses on the entire complement of bacterial types present in each major system. These sample DNA sets from the Wild Reef Exhibit show drastic changes in the diversity of species in all but the Philippines water samples when comparing early to late sample collections. It appears that many species are lost as new bacteria become dominant during the 80 week study describing bacteria in Lake Michigan freshwater succession to more marine-like bacteria in the aquaria water and filter systems. Not surprisingly, the nitrogen cycle related bacteria dominate by the end of the setup period.
Come on out. As usual the setting is very casual and drinks can be ordered at any time before or during the lecture.
FEEL FREE TO COME EARLY AND EAT PRIOR TO LECTURE, OR EAT DURING THE TALK. DOORS OPEN AT 6:00 PM.